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High-throughput Two-photon Microscope Developed for Multiparameter and Quantitative Brain Function Imaging

Large-scale recording of multiple dynamic behaviors and quantitative neurochemical concentrations with high spatiotemporal resolution is critical to understand complex brain functions. Two-photon microscopy (TPM) is the excellent choice for brain function imaging because of its high resolution, deep tissue penetration, and favorable biosafety. However, current TPM is limited by a restricted field-